101. Recombinant DNA Here, however, we shall examine how cloning is done in vivo. But here let usexamine an example of cloning using E. coli as the host and a plasmid as http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html

Recombinant DNA and Gene Cloning Index to this page An Overview Plasmids An Example pAMP ... Recombinant DNA products for human therapy Recombinant DNA is DNA that has been created artificially. DNA from two or more sources is incorporated into a single recombinant molecule. Making Recombinant DNA (rDNA): An Overview Treat DNA from both sources with the same restriction endonuclease (BamHI in this case). BamHI cuts the same site on both molecules 5' GGATCC 3' 3' CCTAGG 5' The ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end. In this case, both DNA preparations have complementary sticky ends and thus can pair with each other when mixed. DNA ligase covalently links the two into a molecule of recombinant DNA To be useful , the recombinant molecule must be replicated many times to provide material for analysis, sequencing , etc. Producing many identical copies of the same recombinant molecule is called cloning . Cloning can be done in vitro , by a process called the polymerase chain reaction PCR ). Here, however, we shall examine how cloning is done

102. CNN.com - Bush Prods Senate To Adopt Ban On All Cloning - April 11, 2002 CNN http://cnn.com/2002/HEALTH/04/10/bush.cloning/index.html

MAIN PAGE WORLD U.S. WEATHER ... ABOUT US/HELP CNN TV what's on show transcripts CNN Headline News CNN International ... askCNN EDITIONS CNN.com Asia CNN.com Europe CNNenEspanol.com CNNArabic.com ... set your edition Languages Spanish Portuguese German Italian Korean Arabic Japanese Time, Inc. Time.com People Fortune EW InStyle Business 2.0 Bush prods Senate to adopt ban on all cloning Bush: "Life is a creation, not a commodity." From Major Garrett CNN White House Correspondent WASHINGTON (CNN) President Bush on Wednesday pressed the Senate to forbid the cloning of human embryos either for research or reproductive purposes, saying any reason for human cloning would be unethical. "Anything other than a total ban on human cloning would be unethical," he said in a speech at the White House. "Research cloning would contradict the most fundamental principle of medical ethics that no human life should be exploited or extinguished for the benefit of another." Bush issued a call last year against cloning human embryos, and Wednesday he built on that call, arguing that other forms of biomedical research offer greater promise without the moral objections raised by cloning. EXTRA INFORMATION Flash Animation: The cloning process Flash Animation: Cloning for treatments Timeline: A chronology of cloning Bush endorsed legislation sponsored by Sens. Sam Brownback, R-Kansas, and Mary Landrieu, D-Louisiana, that would make it a federal crime to create a cloned human embryo. The bill has 29 Senate co-sponsors but Landrieu, running for re-election this year, is the only Democrat to sign onto the measure.

103. Cloning Issues House Passes NRLCBacked Bill to Ban the cloning of Human Embryos, NRLC Responseto Phony Ban on Human cloning Introduced in Congress Feb. 5, 2003 http://www.nrlc.org/Killing_Embryos/Index.html

Help Fight Human Cloning Media Myths! click here Key Documents: Human Cloning Legislation in Congress: Misconceptions and Realities. Questions and Answers on Human Cloning, by Congressman Dave Weldon, M.D. April, 2005 Letter in which Secretary of Health and Human Services Tommy Thompson says he will recommend veto of any bill to authorize human cloning for research Justice Department testimony on how human cloning legislation would be enforced (pdf file) - N R LC Launches New Radio Ads in Eight States, Urging Calls to Senators in Support of Brownback-Landrieu Bill ... The Amazing Vanishing Embryo Trick Federal Legislation Links Le gislative Action Center Congressional News Killing Human Embryos ... Other Congressional Issues KILLING HUMAN EMBRYOS (Human Cloning and Related Issues) For more information: www.stemcellresearch.org and www.cloninginformation.org Human Cloning Legislation in Congress: Misconceptions and Realities. Updated September 13, 2005, this factsheet refutes misconceptions about human cloning , and cloning-related legislation in Congress, that are being disseminated by some advocates of human cloning for research and by some journalists To view or download a PDF version of this document, click

104. CNN.com - British Panel Recommends Human Cloning For Cell Research - August 16, CNN http://www.cnn.com/2000/HEALTH/08/16/embryo.cloning.ap/index.html

health Editions myCNN Video ... Feedback CNN Sites CNN CNN Europe CNNfn CNNSI myCNN CNNfyi AllPolitics Languages Search CNN.com CNNSI.com CNNfn.com The Web HEALTH TOP STORIES New treatments hold out hope for breast cancer patients MORE TOP STORIES Thousands dead in India; quake toll rapidly rising Israelis, Palestinians make final push before Israeli election Davos protesters confront police MORE ... MORE MARKETS 4:30pm ET, 4/16 DJIA NAS SPORTS Jordan says farewell for the third time ... LOCAL EDITIONS: CNN.com Europe change default edition MULTIMEDIA: video video archive audio multimedia showcase ... more services E-MAIL: Subscribe to one of our news e-mail lists Enter your address: DISCUSSION: chat feedback CNN WEB SITES: CNNfyi.com CNN.com Europe AsiaNow Spanish ... Korean Headlines TIME INC. SITES: Go To ... Time.com People Money Fortune EW CNN NETWORKS: CNN anchors transcripts Turner distribution SITE INFO: help contents search ad info ... jobs WEB SERVICES: British panel recommends human cloning for cell research LONDON (AP) A panel of experts urged the government to allow human cloning for scientific study of transplants, a recommendation that, if approved, would make Britain the first country in the world to authorize human cloning for any purpose. In calling for Britain to amend its ban on human cloning, the government-commissioned panel said scientists should be allowed to create cloned embryos to study the manufacture of cells and tissues for transplant.

105. Ad Hoc Committee On An International Convention Against The convention against the reproductive cloning of human beings. convention onthe prohibition of all forms of human cloning (A/58/73 (E, F, S, R, C, http://www.un.org/law/cloning/

106. CNN.com - Bioethicist Says Human Cloning Is Scary - July 17, 2002 CNN http://cnn.com/2001/HEALTH/08/06/kagan.caplan.cnna/index.html

MAIN PAGE WORLD U.S. WEATHER ... ABOUT US/HELP CNN TV what's on show transcripts CNN Headline News CNN International ... askCNN EDITIONS CNN.com Asia CNN.com Europe CNNenEspanol.com CNNArabic.com ... set your edition Languages Spanish Portuguese German Italian Korean Arabic Japanese Time, Inc. Time.com People Fortune EW InStyle Business 2.0 Bioethicist says human cloning is scary Editor's Note: CNN Access is a regular feature on CNN.com providing interviews with newsmakers from around the world. CNN anchor Daryn Kagan talked Monday with Art Caplan, Center for Bioethics at the University of Pennsylvania, about the ethics of human cloning. KAGAN: The first thing you've got to think is this is scary stuff, 200 people, 200 potential cloned babies running around out there. CAPLAN: It is scary. Dr. [Panos] Zavos and his group have been kind of the high-flying showbiz operators of cloning. They keep saying they're going to do this. I have to say, if you looked at the animal work that's been done and the people who really know this procedure of cloning, that is, veterinarians who try it in animals, the procedure is just not safe. And while Zavos and his group keep saying [they've] got something different, no one seems to know what it is. I'm really worried that what they're going to do here is make a dead or deformed baby, not a healthy one.

107. GSM Cloning GSM cloning. History On April 13, 1998, the Smartcard Developer Association Overthe-air cloning In our original announcement, we noted that we could http://www.isaac.cs.berkeley.edu/isaac/gsm.html

108. GSM Cloning Here is some information on our GSM cloning results, starting at a very highlevel, and moving on eventually to detailed technical information, http://www.isaac.cs.berkeley.edu/isaac/gsm-faq.html

109. Science Explained - Your Review Of Science You Want To Know! Online science lessons on a wide variety of subjects, including the cloning of Dolly the sheep, the Montserrat volcano, water on the Moon, Martian fossils, and the chicken flu. http://www.synapses.co.uk/science/index.html

Welcome to Science Explained On these pages you can learn about the exciting things going on in the world of science. Anyone interested in expanding their knowledge of nature and the universe will find this website useful. Hi! I'm Dr Jamie Love. (Yes, I'm really called "Dr Love", so stop laughing!) I have a PhD in Biochemistry and Molecular Biology. I also have many years of experience teaching a wide variety of science subjects, from Astronomy to Zoology, to a wide variety of students, from teenagers to medical students. I created Science Explained to address the needs of those who want a deeper understanding of the world of science. Here I've posted "science features" which highlight a specific topic in the news. I assume no prior knowledge so no one will be left behind. After reading each feature you should have a deep enough understanding of the science and technology that you should be able to intelligently discuss the research, sometimes even the specifics of the research paper itself! I also offer free, monthly

110. Cloning Q&A: Biblical And Scientific Information On Cloning What is cloning? Is it Biblically right or wrong, and how is Genesis creationrelevant? cloning Exposing Flaws in the Preembryoembryo distinction and http://www.answersingenesis.org/home/area/faq/cloning.asp

@import url("/styles/default.css"); Answers in Genesis: Upholding the Authority of the Bible from the Very First Verse Upholding the Authority of the Bible from the Very First Verse Australia Canada New Zealand South Africa ... Other Countries ... Country: Select your country Language: English Good news About us Contact us ... Print-Friendly Country Select Please select your country to get all the benefits of this site: Australia Canada New Zealand South Africa United Kingdom United States Other countries... Afghanistan Albania Algeria Andorra Angola Antigua and Barbuda Argentina Armenia Aruba Australia Austria Azerbaijan Azores Bahamas Bahrain Balearic Islands Bangladesh Barbados Belarus Belgium Belize Benin Bhutan Bolivia Bosnia Herzegovina Botswana Brazil Brunei Bulgaria Burkina Faso Burundi Cambodia Cameroon Canada Canary Islands Cape Verde Cayman Islands Central African Republic Chad Chile China Colombia Comoros Congo Costa Rica Cote d'Ivoire Croatia Cuba Cyprus Czech Republic Denmark Djibouti Dominica Dominican Republic East Timor Ecuador Egypt El Salvador Equatorial Guinea Eritrea Estonia Ethiopia Europe Faroe Islands Fed. States of Micronesia

111. CNN.com - Hatch Supports Some Cloning Research - May 1, 2002 CNN http://cnn.com/2002/ALLPOLITICS/05/01/senate.cloning/index.html

MAIN PAGE WORLD U.S. WEATHER ... ABOUT US/HELP CNN TV what's on show transcripts CNN Headline News CNN International ... askCNN EDITIONS CNN.com Asia CNN.com Europe CNNenEspanol.com CNNArabic.com ... set your edition Languages Spanish Portuguese German Italian Korean Arabic Japanese Time, Inc. Time.com People Fortune EW InStyle Business 2.0 Hatch supports some cloning research From Dana Bash CNN Washington WASHINGTON (CNN) After what he termed "countless hours of study, reflection and prayer," Sen. Orrin Hatch, R-Utah, Tuesday joined a bi-partisan group of senators in sponsoring a bill that would ban most human cloning but allow an exception for research purposes. "I come to this issue with a strong pro-life, pro-family record. But I also strongly believe that a critical part of being pro-life is to support measures that help the living," said Hatch, who was also one of a handful of senators opposed to abortion who supported federal funding for stem cell research. A competing bill, backed by President Bush, would ban all cloning. Hatch said he decided to co-sponsor the measure because he believes cloning for research has promise in finding cures to disease. Other sponsors of the measure include Sens. Ted Kennedy, D-Massachusetts, Dianne Feinstein, D-California, Arlen Specter, R-Pennsylvania and Zell Miller, D-Georgia. The bill would make human cloning a federal crime, with penalties up to $1 million dollars and 10 years in prison. But it would allow research cloning, or what scientists refer to as "regenerative cloning."

112. Reflections On Cloning Human cloning belongs to the eugenics project and is thus subject to all the In the cloning process the basic relationships of the human person are http://www.vatican.va/roman_curia/pontifical_academies/acdlife/documents/rc_pa_a

PONTIFICIA ACADEMIA PRO VITA REFLECTIONS ON CLONING Table of Contents Historical background The biological facts Ethical problems connected with human cloning Human rights and freedom of research HISTORICAL BACKGROUND Advances in knowledge and related developments in the procedures of molecular biology, genetics and artificial fertilization have long made it possible to experiment with and successfully achieve the cloning of plants and animals. Since the '30s experiments have been made in producing identical individuals by artificial twin splitting, a procedure which can be improperly called cloning. The practice of twin splitting in the zootechnical field has been spreading in experimental barns as an incentive to the multiple production of select exemplars. In 1993 Jerry Hall and Robert Stilmann of George Washington University published data concerning the twin splitting they performed on human embryos of two, four and eight embryoblasts. These experiments were conducted without the prior consent of the appropriate Ethics Committee and were published, according to the authors, in order to stimulate the ethical debate. The news published in the journal Nature

113. CNN.com - Bush Praises House For Human Cloning Ban - Feb. 27, 2003 CNN http://cnn.com/2003/ALLPOLITICS/02/27/bush.human.cloning/index.html

The Web CNN.com Home Page World U.S. Weather ... Special Reports SERVICES Video E-mail Newsletters CNNtoGO SEARCH Web CNN.com Bush praises House for human cloning ban Story Tools RELATED How is cloning done? Chronology of cloning Cloning for treatments WASHINGTON (CNN) President Bush Thursday applauded the House of Representatives for passing legislation banning human cloning, and he urged the Senate to follow suit. "Like most Americans, I believe human cloning is deeply troubling, and I strongly support efforts by Congress to ban all human cloning," Bush said in a written statement. "We must advance the promise and cause of medical science, including through ethical stem cell research, yet we must do so in ways that respect human dignity and help build a culture of life. I urge the Senate to act quickly on legislation banning all human cloning." Earlier in the day, the House, in a 241-155 vote, passed a bill banning human cloning that would impose a prison sentence of up 10 years for violators and fines beginning at $1 million. The bill says it would be unlawful for anyone "to perform or attempt to perform human cloning; to participate in an attempt to perform human cloning; or to ship or receive for any purpose an embryo produced by human cloning or any product derived from such embryo."

114. Technical Protocols The Joy of cloning. Table of Contents. Purification of DNA. Phenol Extraction Ethanol Precipitation Recombinant DNA Techniques and cloning http://lena.jax.org/~jcs/techniques/techniques.html

Technical Protocols Below is a listing of the protocols that are generally used in the Schimenti Lab. They are extracted from our lab techniques manual, otherwise known as The Joy of Cloning Some protocols are essentially the same as those described in published lab guides (such as the Maniatis book or the "Red Book"). Others come from other people. Original references are cited in some cases. Minimal theory, if any, is given. These are cookbook procedures to have at the bench when doing a protocol. Please contact jcs@aretha.jax.org if you have any better or faster protocols, as we are always looking to do things easier. The Joy of Cloning Table of Contents Purification of DNA Phenol Extraction Ethanol Precipitation Purification of DNA from Gels Low melting temperature agarose, standard method ... Isolation of DNA from Acrylamide Gels DNA Isolation From Tissues Isolation of DNA from Tissues Mouse Tail DNA Prep (the classic) Mouse Brain DNA Prep for Southern Blotting Genotyping Mice by PCR, I ... Genotyping Mice by PCR,III We now use the HotSHOT method...by far the best: Biotechniques 29:52 (2000) From bacteria (plasmids, cosmids)

115. Genetic CloningThe Cure For All Diseases Or Doctors Playing God? The cure for all diseases or doctors playing God? Slideshows on biotechnology. http://agrino.org/shangaris/GenClon/index.htm

Genetic Cloning: The cure for all diseases or doctors playing god? Presented on the 27 th April 2000 in LT3 of The Royal College of Surgeons in Ireland Click here to start Table of Contents Genetics Genetic Cloning The cure for all diseases or doctors playing god? Human Cloning I Human Cloning II ... Prepared and Presented by... Author: Email: shangaris@yahoo.com Home Page: http://www.geocities.com/shangaris Best experienced with Click here to start.  Genetic Cloning.The (Essay)

116. Plasmid Cloning http://www.sumanasinc.com/webcontent/anisamples/molecularbiology/plasmidcloning_

117. The Gene Letter By GeneSage - Archives Twins are feared in many cultures, and sometimes one is killed at birth, does this affect peoples perception of cloning? Gene Letter http://www.genesage.com/professionals/geneletter/archives/twins.html

Browse Current Issue Browse Previous Issues Browse Previous Content Search GeneLetter Archives All GeneLetter Resources Discussions Surveys August 1, 1998 Myths About Twins: Public Perceptions Of Cloning Another theme in public perceptions of cloning is the "good-and-evil twins". Twins are feared in many cultures, and sometimes one is killed at birth. Mythological themes attached to twins include the following: l. A "good twin" must have an "evil" twin counterpart. DR. JEKYLL AND MR. HYDE is an example of the theme, although only one person is involved. 2. Twins struggle for supremacy and inheritance. Examples are Jacob and Esau in the Bible, and Romulus and Remus, the co-founders of Rome. 3. Twins have special powers, which may be evil. Dorothy C. Wertz

118. Cloning Ethics: Informing Others, Before We Go To Far In Cloning Or Banning It cloning Ethics is a thorough project done by a High School Senior. The siteattempts to inform people about cloning so that perhaps a decision can be made http://lt.tripod.com/tr/directory/_h_/members.tripod.com/cloning/

setAdGroup('67.18.104.18'); var cm_role = "live" var cm_host = "tripod.lycos.com" var cm_taxid = "/memberembedded" Search: Lycos Tripod Free Games Share This Page Report Abuse Edit your Site ... Next What does cloning technology have to offer? How could cloning increase the quality of society, family and life? How could it not? What problems could cloning cause? These pages can be viewed with any Browser at any Resolution (even Lynx ). I suggest using a Browser that can handle tables and display images (animated gif format also). Tell me if a page doesn't look right. Site Awards BEST of POD awarded 3/26/98 This page is mirrored at Tripod here Since Monday, March 12, 1998 (when this page was first uploaded) Get the entire website by downloading cloning.zip This site was a project begun by a Senior from Vergennes Union High School Vermont , USA) for his Advanced Placement (AP) Biology Class Return to Science and Technology or Student and Faculty Sites This page was last updated on Tuesday, March 14, 2000.

119. Nat' Academies Press, Scientific And Medical Aspects Of Human Reproductive Cloni Human Reproductive cloning Proposed Activities and Regulatory Context, 7491 (skim) Appendix B Animal Reproductive cloning Data Tables on Reproductive http://www.nap.edu/openbook/0309076374/html/

Read more than 3,000 books online FREE! More than 900 PDFs now available for sale HOME ABOUT NAP CONTACT NAP HELP ... ORDERING INFO Items in cart [0] TRY OUR SPECIAL DISCOVERY ENGINE Questions? Call 888-624-8373 Scientific and Medical Aspects of Human Reproductive Cloning (2002) Committee on Science, Engineering, and Public Policy ( COSEPUP Board on Life Sciences ( BLS Find More Like This Book Research ... Dashboard NEW! BUY This Book CHAPTER SELECTOR: Openbook Linked Table of Contents Front Matter, pp. i-xxii Executive Summary, pp. 1-18 1. Introduction, pp. 19-23 2. Cloning: Definitions and Applications, pp. 24-38 3. Animal Cloning, pp. 39-60 4. Assisted Reproductive Technology, pp. 61-73 5. Human Reproductive Cloning: Proposed Activities and Regu..., pp. 74-91 6. Findings and Recommendations, pp. 92-100 Appendix A: Panel and Staff Biographical Information, pp. 101-110 Appendix B: Animal Reproductive Cloning Data Tables on Repro..., pp. 111-143 Appendix C: Workshop Agenda and Speaker Biographical Inform..., pp. 144-153 Appendix D: Bibliography, pp. 154-258

120. Hello Dolly! sought in cloning saga. Snookered again truth behind the DNA mystery Revealed!Dolly NOT a perfect replica Found! cloning instruction sheet http://whyfiles.org/034clone/

POSTED 6 MARCH 1997 Scientist in Scotland grows " impossible " sheep from one cell of another sheep. sought in cloning saga. Should there be some LIMITS on science? Lost, then found again! Elvis had himself CLONED! This accounts for simultaneous, multiple sightings. sheep that make medicine There are documents. Glossary Bibliography Credits Search

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