Recombinant DNA Here, however, we shall examine how cloning is done in vivo. But here let usexamine an example of cloning using E. coli as the host and a plasmid as http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html
Extractions: Recombinant DNA and Gene Cloning Index to this page Recombinant DNA is DNA that has been created artificially. DNA from two or more sources is incorporated into a single recombinant molecule. Treat DNA from both sources with the same restriction endonuclease (BamHI in this case). BamHI cuts the same site on both molecules 5' GGATCC 3' 3' CCTAGG 5' The ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end. In this case, both DNA preparations have complementary sticky ends and thus can pair with each other when mixed. DNA ligase covalently links the two into a molecule of recombinant DNA To be useful , the recombinant molecule must be replicated many times to provide material for analysis, sequencing , etc. Producing many identical copies of the same recombinant molecule is called cloning . Cloning can be done in vitro , by a process called the polymerase chain reaction PCR ). Here, however, we shall examine how cloning is done
Extractions: WASHINGTON (CNN) President Bush on Wednesday pressed the Senate to forbid the cloning of human embryos either for research or reproductive purposes, saying any reason for human cloning would be unethical. "Anything other than a total ban on human cloning would be unethical," he said in a speech at the White House. "Research cloning would contradict the most fundamental principle of medical ethics that no human life should be exploited or extinguished for the benefit of another." Bush issued a call last year against cloning human embryos, and Wednesday he built on that call, arguing that other forms of biomedical research offer greater promise without the moral objections raised by cloning. EXTRA INFORMATION Flash Animation: The cloning process Flash Animation: Cloning for treatments Timeline: A chronology of cloning Bush endorsed legislation sponsored by Sens. Sam Brownback, R-Kansas, and Mary Landrieu, D-Louisiana, that would make it a federal crime to create a cloned human embryo. The bill has 29 Senate co-sponsors but Landrieu, running for re-election this year, is the only Democrat to sign onto the measure.
Cloning Issues House Passes NRLCBacked Bill to Ban the cloning of Human Embryos, NRLC Responseto Phony Ban on Human cloning Introduced in Congress Feb. 5, 2003 http://www.nrlc.org/Killing_Embryos/Index.html
Extractions: April, 2005 Letter in which Secretary of Health and Human Services Tommy Thompson says he will recommend veto of any bill to authorize human cloning for research Justice Department testimony on how human cloning legislation would be enforced (pdf file) - N R LC Launches New Radio Ads in Eight States, Urging Calls to Senators in Support of Brownback-Landrieu Bill ... The Amazing Vanishing Embryo Trick Federal Legislation Links Le gislative Action Center Congressional News Killing Human Embryos ... Other Congressional Issues
Extractions: WEB SERVICES: LONDON (AP) A panel of experts urged the government to allow human cloning for scientific study of transplants, a recommendation that, if approved, would make Britain the first country in the world to authorize human cloning for any purpose. In calling for Britain to amend its ban on human cloning, the government-commissioned panel said scientists should be allowed to create cloned embryos to study the manufacture of cells and tissues for transplant.
Extractions: Languages Spanish Portuguese German Italian Korean Arabic Japanese Time, Inc. Time.com People Fortune EW InStyle Business 2.0 Editor's Note: CNN Access is a regular feature on CNN.com providing interviews with newsmakers from around the world. CNN anchor Daryn Kagan talked Monday with Art Caplan, Center for Bioethics at the University of Pennsylvania, about the ethics of human cloning. KAGAN: The first thing you've got to think is this is scary stuff, 200 people, 200 potential cloned babies running around out there. CAPLAN: It is scary. Dr. [Panos] Zavos and his group have been kind of the high-flying showbiz operators of cloning. They keep saying they're going to do this. I have to say, if you looked at the animal work that's been done and the people who really know this procedure of cloning, that is, veterinarians who try it in animals, the procedure is just not safe. And while Zavos and his group keep saying [they've] got something different, no one seems to know what it is. I'm really worried that what they're going to do here is make a dead or deformed baby, not a healthy one.
GSM Cloning GSM cloning. History On April 13, 1998, the Smartcard Developer Association Overthe-air cloning In our original announcement, we noted that we could http://www.isaac.cs.berkeley.edu/isaac/gsm.html
GSM Cloning Here is some information on our GSM cloning results, starting at a very highlevel, and moving on eventually to detailed technical information, http://www.isaac.cs.berkeley.edu/isaac/gsm-faq.html
Science Explained - Your Review Of Science You Want To Know! Online science lessons on a wide variety of subjects, including the cloning of Dolly the sheep, the Montserrat volcano, water on the Moon, Martian fossils, and the chicken flu. http://www.synapses.co.uk/science/index.html
Extractions: Hi! I'm Dr Jamie Love. (Yes, I'm really called "Dr Love", so stop laughing!) I have a PhD in Biochemistry and Molecular Biology. I also have many years of experience teaching a wide variety of science subjects, from Astronomy to Zoology, to a wide variety of students, from teenagers to medical students. I created Science Explained to address the needs of those who want a deeper understanding of the world of science. Here I've posted "science features" which highlight a specific topic in the news. I assume no prior knowledge so no one will be left behind. After reading each feature you should have a deep enough understanding of the science and technology that you should be able to intelligently discuss the research, sometimes even the specifics of the research paper itself!
Cloning Q&A: Biblical And Scientific Information On Cloning What is cloning? Is it Biblically right or wrong, and how is Genesis creationrelevant? cloning Exposing Flaws in the Preembryoembryo distinction and http://www.answersingenesis.org/home/area/faq/cloning.asp
Extractions: @import url("/styles/default.css"); Upholding the Authority of the Bible from the Very First Verse Country: Select your country Language: English Good news About us Contact us ... Print-Friendly Please select your country to get all the benefits of this site: Australia Canada New Zealand South Africa United Kingdom United States Other countries... Afghanistan Albania Algeria Andorra Angola Antigua and Barbuda Argentina Armenia Aruba Australia Austria Azerbaijan Azores Bahamas Bahrain Balearic Islands Bangladesh Barbados Belarus Belgium Belize Benin Bhutan Bolivia Bosnia Herzegovina Botswana Brazil Brunei Bulgaria Burkina Faso Burundi Cambodia Cameroon Canada Canary Islands Cape Verde Cayman Islands Central African Republic Chad Chile China Colombia Comoros Congo Costa Rica Cote d'Ivoire Croatia Cuba Cyprus Czech Republic Denmark Djibouti Dominica Dominican Republic East Timor Ecuador Egypt El Salvador Equatorial Guinea Eritrea Estonia Ethiopia Europe Faroe Islands Fed. States of Micronesia
Extractions: WASHINGTON (CNN) After what he termed "countless hours of study, reflection and prayer," Sen. Orrin Hatch, R-Utah, Tuesday joined a bi-partisan group of senators in sponsoring a bill that would ban most human cloning but allow an exception for research purposes. "I come to this issue with a strong pro-life, pro-family record. But I also strongly believe that a critical part of being pro-life is to support measures that help the living," said Hatch, who was also one of a handful of senators opposed to abortion who supported federal funding for stem cell research. A competing bill, backed by President Bush, would ban all cloning. Hatch said he decided to co-sponsor the measure because he believes cloning for research has promise in finding cures to disease. Other sponsors of the measure include Sens. Ted Kennedy, D-Massachusetts, Dianne Feinstein, D-California, Arlen Specter, R-Pennsylvania and Zell Miller, D-Georgia. The bill would make human cloning a federal crime, with penalties up to $1 million dollars and 10 years in prison. But it would allow research cloning, or what scientists refer to as "regenerative cloning."
Reflections On Cloning Human cloning belongs to the eugenics project and is thus subject to all the In the cloning process the basic relationships of the human person are http://www.vatican.va/roman_curia/pontifical_academies/acdlife/documents/rc_pa_a
Extractions: HISTORICAL BACKGROUND Advances in knowledge and related developments in the procedures of molecular biology, genetics and artificial fertilization have long made it possible to experiment with and successfully achieve the cloning of plants and animals. Since the '30s experiments have been made in producing identical individuals by artificial twin splitting, a procedure which can be improperly called cloning. The practice of twin splitting in the zootechnical field has been spreading in experimental barns as an incentive to the multiple production of select exemplars. In 1993 Jerry Hall and Robert Stilmann of George Washington University published data concerning the twin splitting they performed on human embryos of two, four and eight embryoblasts. These experiments were conducted without the prior consent of the appropriate Ethics Committee and were published, according to the authors, in order to stimulate the ethical debate. The news published in the journal Nature
Extractions: Chronology of cloning Cloning for treatments WASHINGTON (CNN) President Bush Thursday applauded the House of Representatives for passing legislation banning human cloning, and he urged the Senate to follow suit. "Like most Americans, I believe human cloning is deeply troubling, and I strongly support efforts by Congress to ban all human cloning," Bush said in a written statement. "We must advance the promise and cause of medical science, including through ethical stem cell research, yet we must do so in ways that respect human dignity and help build a culture of life. I urge the Senate to act quickly on legislation banning all human cloning." Earlier in the day, the House, in a 241-155 vote, passed a bill banning human cloning that would impose a prison sentence of up 10 years for violators and fines beginning at $1 million. The bill says it would be unlawful for anyone "to perform or attempt to perform human cloning; to participate in an attempt to perform human cloning; or to ship or receive for any purpose an embryo produced by human cloning or any product derived from such embryo."
Technical Protocols The Joy of cloning. Table of Contents. Purification of DNA. Phenol Extraction Ethanol Precipitation Recombinant DNA Techniques and cloning http://lena.jax.org/~jcs/techniques/techniques.html
Extractions: Below is a listing of the protocols that are generally used in the Schimenti Lab. They are extracted from our lab techniques manual, otherwise known as The Joy of Cloning Some protocols are essentially the same as those described in published lab guides (such as the Maniatis book or the "Red Book"). Others come from other people. Original references are cited in some cases. Minimal theory, if any, is given. These are cookbook procedures to have at the bench when doing a protocol. Please contact jcs@aretha.jax.org if you have any better or faster protocols, as we are always looking to do things easier. From bacteria (plasmids, cosmids)
The Gene Letter By GeneSage - Archives Twins are feared in many cultures, and sometimes one is killed at birth, does this affect peoples perception of cloning? Gene Letter http://www.genesage.com/professionals/geneletter/archives/twins.html
Extractions: August 1, 1998 Myths About Twins: Public Perceptions Of Cloning Another theme in public perceptions of cloning is the "good-and-evil twins". Twins are feared in many cultures, and sometimes one is killed at birth. Mythological themes attached to twins include the following: l. A "good twin" must have an "evil" twin counterpart. DR. JEKYLL AND MR. HYDE is an example of the theme, although only one person is involved. 2. Twins struggle for supremacy and inheritance. Examples are Jacob and Esau in the Bible, and Romulus and Remus, the co-founders of Rome. 3. Twins have special powers, which may be evil. Dorothy C. Wertz
Extractions: Search: Lycos Tripod Free Games Share This Page Report Abuse Edit your Site ... Next These pages can be viewed with any Browser at any Resolution (even Lynx ). I suggest using a Browser that can handle tables and display images (animated gif format also). Tell me if a page doesn't look right. Site Awards BEST (when this page was first uploaded) Get the entire website by downloading cloning.zip This site was a project begun by a Senior from Vergennes Union High School Vermont , USA) for his Advanced Placement (AP) Biology Class Return to Science and Technology or Student and Faculty Sites This page was last updated on Tuesday, March 14, 2000.
Extractions: Openbook Linked Table of Contents Front Matter, pp. i-xxii Executive Summary, pp. 1-18 1. Introduction, pp. 19-23 2. Cloning: Definitions and Applications, pp. 24-38 3. Animal Cloning, pp. 39-60 4. Assisted Reproductive Technology, pp. 61-73 5. Human Reproductive Cloning: Proposed Activities and Regu..., pp. 74-91 6. Findings and Recommendations, pp. 92-100 Appendix A: Panel and Staff Biographical Information, pp. 101-110 Appendix B: Animal Reproductive Cloning Data Tables on Repro..., pp. 111-143 Appendix C: Workshop Agenda and Speaker Biographical Inform..., pp. 144-153 Appendix D: Bibliography, pp. 154-258
Hello Dolly! sought in cloning saga. Snookered again truth behind the DNA mystery Revealed!Dolly NOT a perfect replica Found! cloning instruction sheet http://whyfiles.org/034clone/