1. Molecular Expressions Microscopy Primer: Electron Microscopy Interactive Java Tu This interactive tutorial explores imaging of a variety of specimens in a Scanning Electron Microscope. http://micro.magnet.fsu.edu/primer/java/electronmicroscopy/magnify1/index.html

Microscopy Primer Light and Color Microscope Basics Special Techniques ... Home The Galleries: Photo Gallery Silicon Zoo Pharmaceuticals Chip Shots ... Movie Gallery Interactive Java Tutorials Virtual Scanning Electron Microscopy We have teamed up with award-winning electron microscopist Dr. Dennis Kunkel to produce a series of interactive Java tutorials that explore various aspects of virtual Scanning Electron Microscopy (vSEM). Use the tutorial below to discover how specimens appear when magnified in the virtual SEM. Interactive Java Tutorial ATTENTION Our servers have detected that your web browser does not have the Java Virtual Machine installed or it is not functioning properly. Please install this software in order to view our interactive Java tutorials. You may download the necessary software by clicking on the "Get It Now" button below. When the tutorial is first loaded, the specimen is out of focus and brightness and contrast are not optimized. Use the Focus slider to achieve focus and the Contrast and Brightness sliders to optimize specimen appearance. Next, use the

2. Electron Microscopy electron microscopy. Return to the histotechnology menu. The following grayscale electron micrographic images are available file sizes average 100 to 160k http://library.med.utah.edu/WebPath/HISTHTML/EM/EM.html

Electron Microscopy Return to the histotechnology menu. The following grayscale electron micrographic images are available [file sizes average 100 to 160k]: Normal Structures Structure Description Neutrophil Normal polymorphonuclear leukocyte Lymphocyte Normal lymphocyte Plasma cell Normal plasma cell with prominent cytoplasmic smooth endoplasmic reticulum Macrophage Normal macrophage with oblong nucleus, nucleolus, and cytoplasm with a variety of inclusions Platelets Normal platelets Mitochondria Happy mitochondria within a cell Skeletal muscle Normal skeletal muscle, normal (inset shows a single sarcomere with dark Z discs, and a central M line, with thin actin and thick myosin filaments) Collagen Normal collagen fibrils Cilia Normal cilia of respiratory tract epithelium Miscellaneous Findings Structure Description Amyloid Amyloid fibrils, with inset demonstrating array of fibrils Adrenoleukodystrophy Adrenoleukodystrophy, adrenal gland, with long parallel inclusions of long chain fatty acids Birbeck granules Birbeck granules (inset shows several at higher magnification) from a case of histiocytosis X Histiocytosis X Cell with a clefted nucleus from a case of histiocytosis X Bacteria Pseudomonas bacteria Cryptosporidia Cryptosporidia along small intestinal brush border Herpes simplex virus Herpes simplex virus in brain, viral particles (inset) in nucleus

3. Scanning Electron Microscopy What is the SEM? Info on the Scanning Electron Microscope includes a picture of the SEM. How does the SEM work? This link will allow you to learn about the http://mse.iastate.edu/microscopy/home.html

Info on Submitting Samples SEM What is the SEM? Info on the Scanning Electron Microscope -includes a picture of the SEM. How does the SEM work? This link will allow you to learn about the SEM at the level of your choice. Picture Library SEM Images Includes pictures submitted by students from around the state of Iowa. Other Interesting Pages Iowa State University Homepage Materials Science and Engineering Homepage Other interesting pages This site is listed in the BBC Education ... on the Net are only a click away! Questions and Comments? This page is maintained by Josh Klesel and the Material Science and Engineering Department at Iowa State University. If you have any comments or questions, send email to jklesel@iastate.edu or Prof. Scott Chumbley

4. The World-Wide Web Virtual Library: Microscopy Covers all aspects of light microscopy, electron microscopy and other forms of microscopy. http://www.ou.edu/research/electron/www-vl/

Microscopy This page of the WWW-Virtual Library covers all aspects of light microscopy, electron microscopy and other forms of microscopy. Topics can be viewed individually, or as part of one long list . The newest links are periodically incorporated as part of the main list, and are also presented in my list of updates . The links on this site were last checked using Cyberspider on November 28, 2000, when all 2483 links were evaluated. Please enjoy. Internet Resources Reference Sites Laboratories, Organizations and People Equipment and Technology ... Vendors and Other Selections: Internet Resources: Listservs Search Engines and Web Sites Usenet Newsgroups Reference Sites: WWW-VL Related Sites Reference Materials Laboratories, Organizations and People: Organizations Labs Microscopist's Home Pages Institutes ... Museums Equipment and Technology: Atomic Force Microscopy (AFM) Confocal Laser Scanning Microscopy (CLSM) Ion Beams Nearfield Scanning Optical Microscopy (NSOM) ... X-ray Microscopy Applications and Topics: Biologically-Oriented Sites Material Sciences-Oriented Sites Math-related Sites Medically-Oriented Sites ... Virology Sites General and Education: Conferences Education Sites General Image Libraries ... Journals Techniques: Technique Pages Vendors and Other: Vendors Unclassified Sites Some of the links in this site were developed in cooperation with MicroWorld Resources and News . The WWW Virtual Library in Microscopy is sponsored by the Samuel Roberts Electron Microscopy Laboratory and the University of Oklahoma . Please send inquiries, URLs, corrections through our

5. Electron Microscopy - SEM - TEM - SPI Supplies® SourceBook Catalog - Microscope SPI Supplies OnLine Catalog Table of Contents of microscopy supplies lists products by category, from instruments of sample preparation, including sputter http://www.2spi.com/catalog.html

In Memory of Charles A. Garber, PhD. SPI Supplies SourceBook Everything you need for a quality microscopy laboratory Chinese Russian Spanish Table of Contents: Navigation on a website is always one of the most important considerations as to whether any particular viewer will ever bookmark and/or return on some date in the future. We offer our website visitors and customers an index below. And we offer them the SPI Navigator Index box which is found in the lower right hand corner of each page and in to the right of this text. We also recommend following the MicroChuck Microscopy Blog for timely and further information about various microscopy topics facing those working in microscopy today. Some of the topics might be seen as "controversial". Any reader of the blog is free to make their own postings and express their own opinions being covered. We welcome contrary points of view. Instruments: Sputter / Carbon Coaters for SEM/EDS applications Plasma Etchers for failure analysis and surface cleaning Plasma Cleaners for TEM Specimen Holders and Rods for contamination rate reduction Chromium Coaters for FESEM Applications Osmium Plasma Coater for Ultimate SEM Metal Coatings Critical Point Dryers and Supplies Ultrasonic Disc Cutters / Grinders and Supplies Tissue Processors for thin foil preparation Water Chillers / Recirculators Vacuum Pumps Light Microscopes, Research Type

6. Oxford Journals | Life Sciences | Journal Of Electron Microscopy The Journal of electron microscopy is the official journal of the Japanese Society of Microscopy, the second largest society of microscopy in the world. http://jmicro.oxfordjournals.org/

@import "/resource/css/main.css"; @import "/resource/css/homepage.css"; @import "/resource/css/journal.css"; @import "/resource/css/jmicro.css"; @import "/resource/css/progress.css"; Skip Navigation Oxford Journals Contact Us My Basket ... My Account Journal of Electron Microscopy About This Journal Contact This Journal Subscriptions Current Issue ... Life Sciences Journal of Electron Microscopy Read This Journal View Current Issue (Volume 56 Issue 6 December 2007) Advance Access Browse the Archive View all Supplements The Journal of Electron Microscopy is the official journal of the Japanese Society of Microscopy, the second largest society of microscopy in the world. The journal is an international forum, open to all scientists in the field, for publishing the best research in advanced electron microscopy and new scanning probe microscopy. To ensure this policy, the journal has appointed eminent scientists from around the world to be Regional Editors. The Journal of Electron Microscopy publishes six issues a year, and papers cover the application of advanced microscopy in diverse fields. Articles cover theories, methods, techniques, and instrumentation, as well as their applications to life and material sciences.

7. TEM Basics This is a set of resources designed to accompany an introductory course on transmission electron microscopy. The level is appropriate for students with an http://www.matter.org.uk/tem/

MATTER TEM Site Map Help ... MATTER TEM Basics Transmission Electron Microscopy (TEM) Storyboard: Peter Goodhew University of Liverpool Based on Textbook: Transmission Electron Microscopy - Basics by D.B.Williams and C.B.Carter Topic Williams and Carter A. Electron Scattering Sec. 2.6; p26 B. Electron atom interactions Sec 2.11; p30 B1. Introduction B2. Inelastic scattering B3. X-ray and Auger Sec 4.3C; p58 B4. Simulation C. Electron gun C1. Electron sources Sec 5.3A; p72 C2. Gun simulation - introduction Sec 5.3A; p72 C3. Gun simulation Sec 5.3A; p72 D. Diffraction at an aperture Sec. 2.11; p30 E. Resolution of 2 sources

8. Centre Interdepartemental De Microscopie Electronique Welcome to CIME, the central facility and research center in electron microscopy at. Ecole Polytechnique Fédérale de Lausanne http://cimewww.epfl.ch/

C entre I nterdisciplinaire de M icroscopie E lectronique Welcome to CIME, the central facility and research center in electron microscopy at E cole P olytechnique F L ausanne Booking electron microscope periods Proposition projets de semestre section SMX hiver 2006

9. Dennis Kunkel Microscopy - Electron Microscopy Science Stock Photography View scanning electron microscope pictures of interesting creatures and household items. Offers a simulation of using an electron microscope. http://www.denniskunkel.com/

Dennis Kunkel Microscopy, Inc. Science Stock Photography Home Assignment Photography Video and Animation About Dennis Kunkel ... Education Site Quick Search Find images by keyword Advanced Search Latest Scientific Stock Photography Images October 2007 Log In E-mail address: Password: (Forgotten it?) Create an Account (to save a light box) Education Site More Information Site Map How to Use This Site Light Box Information Image Use ... Products Science Store Light Box no images Category Listings Image Category Listings at a glance Algae Arachnids Bacteria Crystals ... Viruses Scientific stock photography library of light microscope pictures and electron microscopy images featuring science and biomedical microscopy photos - over 2,200 light microscopy and electron microscopy images of Algae, Arachnids, Bacteria, Crystals, Fungi, Insects, Invertebrates, Medical, Miscellaneous, Plants, Protozoa, Vertebrates, and Viruses. Biological, medical and general sciences images are available for licensed, rights-managed, non-exclusive use. - Contact us for custom scientific imaging of samples and products. Dennis Kunkel Ph.D has over 35 years of microscopy expertise in biological and medical sciences. Quick Browse By Category Please Select All categories Algae Arachnids Bacteria Crystals Fungi and Slime Molds Insects Medical Misc. Invertebrates

10. Electron Microscopy Sciences, Diatome Diamond Knives, Summers Optical And EMS Co A complete online Product Catalog of chemicals, supplies, accessories, and equipment for Electron and Light Microscopy, Histology, Cell Biology, http://www.emsdiasum.com/

A complete online Product Catalog of chemicals, supplies, accessories, and equipment for Electron and Light Microscopy, Histology, Cell Biology, Neuroscience, and all biological related research fields. At the site, you can find technical tips and recommended articles of interest technical and product data sheets Material Safety Data Sheets , and many revolutionary new products and exclusive items. A complete product catalog of the entire Diatome collection of Diamond knives, tools, and accessories for Electron and Light microscopy for Biological and Materials Science at room and cryo temperatures. Available on-line as well is information on our services, programs, specials, and policies. The complete handling and use technical manual as well as troubleshooting can also be found at our site. The Summers Optical on-line catalog including a complete line of optical cements and adhesives , decementing agents, hardness testers, ultrasonic baths and UV lights as well as technical and transmission data and problem solving can be found at this site. As well as, our unique bonding manual including troubleshooting and charts for how to choose a cement for specific applications and a complete set of

11. Electron Microscopy (Microscopy & Imaging, Cellular Imaging Core, SWEHSC) Other large electron microscopes can be seen at the National Center for electron microscopy at UC Berkley and at the University of Colorado s Boulder http://swehsc.pharmacy.arizona.edu/exppath/micro/em.html

SWEHSC Facility Cores Cellular Imaging Electron Microscopy See also: General Microscopy (Societies, Vendors, WWW, Email Lists) On-line Microscopic Images This page: Historical General EM Information Technique WWW Resources ... Menu Historical The Jerome J. Paulin Electron Microscopy Museum Images of several vintage electron microscopes (and related equipment) from the University of Georgia. John H.L. Watson's recollections Very early transmission electron microscopy in the department of physics, University of Toronto. The author's personal recollections of that time. Early History and Development of the Scanning Electron Microscope Author: Bernie C Breton CUED, Cambridge University, Engineering Department, Scientific Imaging Group. See also an article on Charles Oatley, "Pioneer of scanning electron microscopy" on the same server (author: KCA Smith) 3 Million Volt Electron Microscope This impressive feat of engineering is more recent (mid-1990s) . Osaka University, Japan. Other large electron microscopes can be seen at the National Center for Electron Microscopy at UC Berkley and at the University of Colorado's Boulder Laboratory for 3-D Fine Structure General Electron Microscopy Information Glossary of Electron Microscopy Terms From the University of Nebraska (Lincoln).

12. TEM In Martinsried electron microscopy Yellow Pages The purpose of this service is to provide an extensive collection of links related to electron microscopy inside the World http://www.biochem.mpg.de/baumeister/TEM/home.html

What we do Automated electron tomography (AET) Energy filtered transmission electron microscopy (EFTEM) Take care of our hardware Digital processing of electron microscope images Who we are A list of everybody involved Two different proteins: the chaperone GroEL (left) and the proteasome (right) montage of computer based 3D reconstructions (in different resolutions) with a part of an EM-tomogram What else to click on (external links) You will leave here the internet site of the MPI for Biochemistry Electron Microscopy Yellow Pages MicroWorld Resources and News This site provides information on all types of microscopy, surface science, imaging, image analysis and processing, and microanalytical techniques including x-ray and spectroscopy. A good place to start surfing. ANL Microscopy and Microanalysis WWW Server (Argonne National Laboratory) Microscope 3D Data Base 3-D CryoElectron Microscopy Reconstructions shows images of different virus particles. An almost complete alphabetical listing.

13. NCEM National Center For Electron Microscopy Microscope Scheduling Microscopes and Facilities New Research Publications Microscopy Links. Last updated January 16, 2008. http://ncem.lbl.gov/

About NCEM Staff Becoming an NCEM User ... Microscopy Links Last updated January 23, 2008

14. Botany Online: Microscopy - Electron Microscopy In 1924 L. de BROGLIE discovered the wavecharacter of electron rays thus giving the prerequisite for the construction of the electron microscope. http://www.biologie.uni-hamburg.de/b-online/e03/03e.htm

Electron Microscopy The Transmission Electron Microscope (TEM) The conventional electron microscopy is nowadays called TEM (transmission electron microscopy). We will therefore start with its construction. The ray of electrons is produced by a pin-shaped cathode heated up by current. The electrons are vacuumed up by a high voltage at the anode. The acceleration voltage is between 50 and 150 kV. The higher it is, the shorter are the electron waves and the higher is the power of resolution. But this factor is hardly ever limiting. The power of resolution of electron microscopy is usually restrained by the quality of the lens-systems and especially by the technique with which the preparation has been achieved. Modern gadgets have powers of resolution that range from 0,2 - 0,3 nm. The useful resolution is therefore around 300,000 x. The accelerated ray of electrons passes a drill-hole at the bottom of the anode. Its following way is analogous to that of a ray of light in a light microscope. The lens-systems consist of electronic coils generating an electromagnetic field. The ray is first focused by a condenser. It then passes through the object, where it is partially deflected. The degree of deflection depends on the electron density of the object. The greater the mass of the atoms, the greater is the degree of deflection. Biological objects have only weak contrasts since they consist mainly of atoms with low atomic numbers (C, H, N, O). Consequently it is necessary to treat the preparations with special contrast enhancing chemicals (heavy metals) to get at least some contrast. Additionally they are not to be thicker than 100 nm, because the temperature is raising due to electron absorption. This again can lead to destruction of the preparation. It is generally impossible to examine living objects.

15. Reading On Electron Microscopy The first page of a Discourse on the Electron Microscope. http://acept.asu.edu/PiN/rdg/elmicr/elmicr.shtml

Scanning Electron Microscopy (SEM) Image Formation in the SEM In the SEM, the image is formed and presented by a very fine electron beam, which is focused on the surface of the specimen. The beam is scanned over the specimen in a series of lines and frames called a raster, just like the (much weaker) electron beam in an ordinary television. The raster movement is accomplished by means of small coils of wire carrying the controlling current (the scan coils). A schematic drawing of an electron microscope is shown in Fig. 1. At any given moment, the specimen is bombarded with electrons over a very small area. Several things may happen to these electrons. They may be elastically reflected from the specimen, with no loss of energy. They may be absorbed by the specimen and give rise to secondary electrons of very low energy, together with X- rays. They may be absorbed and give rise to the emission of visible light (an effect known as cathodoluminescence). And they may give rise to electric currents within the specimen. All these effects can be used to produce an image. By far the most common, however, is image formation by means of the low-energy secondary electrons. Figure 1 . Schematic drawing of a scanning electron microscope with secondary electrons forming the images on the TV screen. The SEM does not contain objective, intermediate and projector lenses to magnify the image as in the optical microscope. Instead magnification results from the ratio of the area scanned on the specimen to the area of the television screen. Increasing the magnification in an SEM is therefore achieved quite simply by scanning the electron beam over a smaller area of the specimen.

16. Electron Microscopy For Dummies For Dummies series, this project is an exploration on the basics of electron microscopy in single particle reconstruction, and it s applications to biology http://www.snaggledworks.com/em_for_dummies/

Graphics courtesy of IDG Books In the tradition of IDG Books' " " series, this project is an exploration on the basics of electron microscopy in single particle reconstruction, and it's applications to biology. It is intended for the beginner, who has not previously been exposed in much detail to electron microscopy. Emphasis will be on how a biological sample is prepared for use, how an image is made from that sample, and how the images can be interpreted and manipulated to give structural information about the sample. Not much specific knowledge on the topic discussed is required to understand the material presented here. It is meant for an audience that has some science background, but has not had extensive exposure to the subject of electron microscopy before. The explanations are generally qualitative, although specific values are given where appropriate so that the reader can fit the explanations into a more general scheme of things. This project is a work in progress and is intended as a running notebook of my experiences in how things work in the field of electron microscopy and single particle reconstruction. Therefore, there will never really be a final "authoritative" version. I'm constantly adding new topics, figures, and discussions, as well as using this project as an HTML testbed.

17. Micro-imaging And Analysis This is accomplished by means of graduate level courses in scanning (SEM) and transmission electron microscopy (TEM) as well as training in the use of all http://www.siu.edu/~shops/cem.html

Micro-imaging and Analysis Center The Micro-imaging and Analysis Center at Southern Illinois University is a service unit supported by the Office of Research Development and Administration (ORDA). The MIAC provides service and training in electron and light microscopy. This is accomplished by means of graduate level courses in scanning (SEM) and transmission electron microscopy (TEM) as well as training in the use of all instrumentation in the MIAC. Trained staff will prepare specimens and assist in the viewing of samples and assist in the writing of technical or budgetary proposals related to electron microscopy and the writing of reports for granting agencies. MIAC sponsors workshops on modern research methods in electron microscopy and offers tours of the facility to the university and community. The internal rates are designed to recover all costs associated with expendible supplies, service contracts on major instrumentation and a portion of the salaries of technical personnel. The MIAC is available for outside grant and contract work. Outside rates are negotiated through the Office of Research Development and Administration and are designed to recover all operating expenses as well as an established overhead rate.

18. ImagiNations - What Is Electron Microscopy? electron microscopy involves the study of different specimens by using an electron microscope. Through the use of an electron microscope we can see things http://www.lehigh.edu/~inimagin/whatisem.html

What is Electron Microscopy? Electron Microscopy involves the study of different specimens by using an electron microscope . Through the use of an electron microscope we can see things that we would not normally be able to see with our eyes. So what makes an electron microscope different from the microscopes you use in science class? An electron microscope uses super small particles called electrons to help us magnify what we are looking at. A regular microscope that you can find in your science class room uses light to magnify specimens. What's good about using electrons instead of light to view specimens is that we can magnify an object up to 200,000 times its normal size. Imagine being able to look at a flea as if it were bigger than you! Unlike your regular microscopes in school, we cannot see things in c o l o r using an electron microscope. Also, you can only place inanimate objects in an

19. Analytical And Environmental Electron Microscopy Analytical and Environmental electron microscopy. ESEM, STEM, SEM, TEM. Kansas City, Missouri. http://www.umkc.edu/dentistry/microscopy

Department of Oral Biology Technology and Cores Electron Microscope Laboratory Our laboratory has state-of-the-art instrumentation including Field-Emission Environmental SEM, conventional SEM, and STEM. All our microscopes are equipped with new PGT Imix-PC EDS systems with digital Prism detectors. The laboratory staff is knowledgeable in a wide range of biological and materials research. The Electron Microscope Laboratory performs research in the fields of life and physical sciences for the School of Dentistry, University of Missouri - Kansas City and for those outside the university on a fee basis. We are happy to work with any organization in need of electron microscopy applications, consultation, or training. FEI/Philips XL30 Field-Emission Environmental SEM Philips SEM 515 ... Field-Emission Environmental SEM FEI/Philips XL30 can be used for almost any kind of specimen: 1. Wet specimens: humidity in the specimen chamber can be as high as 100%, making it possible to observe live cells, dissolving and drying processes, etc. (Images) 2. Non-conductive specimens without coating can be observed in "wet" mode with high resolution imaging and EDS capabilities for elemental analysis.

20. Electron Microscopy Group - Biophysical Chemistry - University Of Groningen rugbe2.chem.rug.nl/ Microscopy Ion Scattering Applications Reservations. PENN Regional Nanotechnology Facility http://rugbe2.chem.rug.nl/

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