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         Mullis Kary B:     more detail
  1. The Polymerase Chain Reaction
  2. The Polymerase Chain Reaction (Volume 0)
  3. Dancing Naked in the Mind Field (Bloomsbury Paperbacks) by Kary B. Mullis, 2000-01-21
  4. Biography - Mullis, Kary B(anks) (1944-): An article from: Contemporary Authors Online by Gale Reference Team, 2005-01-01
  5. The Polymerase Chain Reaction by Kary B. Mullis, 2007-01-01
  6. American Biochemists: Isaac Asimov, Linus Pauling, Kary Mullis, Konrad Emil Bloch, Walter Gilbert, Gregory Goodwin Pincus, Stanley B. Prusiner

61. Mullis Autobiography
kary B. mullis – Autobiography. My father Cecil Banks mullis and mother, formerly Bernice Alberta Barker grew up in rural North Carolina in the foothills of
http://www.modares.ac.ir/elearning/mnaderi/Genetic Engineering course II/Pages/K
Kary B. Mullis – Autobiography
Until I was five my immediate family lived near my grandfather's farm where my mother had grown up, and with the exception of a few modern conveniences, had not changed a lot over the years.
My grandfather milked several cows twice a day and supplied the neighbours with dairy products. He liked to go visiting around the county on Saturdays and he also enjoyed the neighbours when they came by once a week with their empty milk jars. He walked them out to their cars and hung over the driver's side window until they drove off. The road was two tire tracks on well mown grass between barbed wire fences, cows off to the right, alfalfa or sometimes corn to the left.
I remember mostly the summers. My mother and aunts presided out on the big screened back porch shelling peas, stringing beans, peeling apples, pears, and peaches. The peaches were peeled with a special machine that had a hand crank and left a spiraling groove on what was left of the peach. The peels went to the pigs. Everything else went into steaming Mason jars which would go down into the earthen floored cellar. Down there in the dark, and it was always a little moist, were spiders in abundance and magnificent biodiversity. My brothers, and my cousins, and I ventured into the cellar once in a while to inspect the sweet potatoes and the hibernating jars. No one wanted to stay there alone ever, and mostly we played in the woods, the swamp, the orchards, the barn, the granary, which had wasps, and the woodshed, which also had wasps and, like the barn, allegedly, snakes.

62. Positively Healthy UK - Gay Men's 360° Health - Popperstoppers - Dr Kary Mullis
Dr. kary B. mullis. On November 30, 1994, I received another letter from Mr. Smith. Dr. kary B. mullis wants to talk to you and your associates,
http://www.posh-uk.org.uk/gmh/kmullis_article1.html
THE MEDICAL ESTABLISHMENT VS. THE TRUTH Home The History Alchemy Registered Enabled ... Dedication Book Excerpt By Dr. Kary Mullis Penthouse Sept. 1998 Dr. Mullis was awarded the 1993 Nobel Prize in Chemistry. This article is excerpted from his forthcoming book, Dancing Naked in the Mind Field , to be published by Pantheon. When I first heard in 1984 that Luc Montagnier of France's Pasteur Institute and Robert Gallo of America's National Institutes of Health had independently discovered that the retrovirus H.I.V. human immunodeficiency virus caused AIDS, I accepted it as just another scientific fact. It was a little out of my field of biochemistry, and these men were specialists in retroviruses. Four years later I was working as a consultant at Specialty Labs in Santa Monica. Specialty was trying to develop a means of using P.C.R. [polymerase chain reaction, a D.N.A.-amplification method conceived by Mullis] to detect retroviruses in the thousands of blood donations received per day by the Red Cross. I was writing a report on our progress for the project sponsor, and I began by stating, "H.I.V. is the probable cause of AIDS."

63. Smithsonian Institution Archives
kary B. mullis received his BS in Chemistry from the Georgia Institute of Technology in 1966 and his Ph.D. in Biochemistry from the University of
http://siarchives.si.edu/research/videohistory_catalog9577.html
dqmcodebase = "../script/" dqmrm="../"; dqm_link="../" Smithsonian Videohistory Collection The History of PCR
(RU 9577) Background The Polymerase Chain Reaction (PCR) technique, invented in 1985 by Kary B. Mullis, allowed scientists to make millions of copies of a scarce sample of DNA. The technique has revolutionized many aspects of current research, including the diagnosis of genetic defects and the detection of the AIDS virus in human cells. The technique is also used by criminologists to link specific persons to samples of blood or hair via DNA comparison. PCR also affected evolutionary studies because large quantities of DNA can be manufactured from fossils containing but trace amounts. Kary Mullis invented the PCR technique in 1985 while working as a chemist at the Cetus Corporation, a biotechnology firm in Emeryville, California. The procedure requires placing a small amount of the DNA containing the desired gene into a test tube. A large batch of loose nucleotides, which link into exact copies of the original gene, is also added to the tube. A pair of synthesized short DNA segments, that match segments on each side of the desired gene, is added. These "primers" find the right portion of the DNA, and serve as starting points for DNA copying. When the enzyme Thermus aquaticus (Taq) is added, the loose nucleotides lock into a DNA sequence dictated by the sequence of that target gene located between the two primers. The test tube is heated, and the DNA's double helix separates into two strands. The DNA sequence of each strand of the helix is thus exposed and as the temperature is lowered the primers automatically bind to their complementary portions of the DNA sample. At the same time, the enzyme links the loose nucleotides to the primer and to each of the separated DNA strands in the appropriate sequence. The complete reaction, which takes approximately five minutes, results in two double helices containing the desired portion of the original. The heating and cooling is repeated, doubling the number of DNA copies. After thirty to forty cycles are completed a single copy of a piece of DNA can be multiplied to hundreds of millions.

64. MSN Encarta - Mullis, Kary B.
Translate this page mullis, kary B. (Lenoir, North Carolina 1944), biochimico americano. Altre risorse di Encarta. Cerca in Encarta mullis, kary B.
http://it.encarta.msn.com/encyclopedia_761582668/Mullis_Kary_B.html
  • MSN Home My MSN Hotmail Shopping ... Migliora la tua esperienza con Encarta Cerca in Encarta
    Articolo riservato agli iscritti MSN Encarta Premium: Puoi consultare questo e altri 30.000 articoli, un atlante interattivo, un dizionario bilingue e altre risorse, effettuando l'iscrizione a MSN Encarta Premium per €29,95 all'anno. Per saperne di pi¹. L'articolo ¨ disponibile solo per gli utenti iscritti a MSN Encarta Premium. Gi  iscritto? Per accedere fare clic sul pulsante Accedi in alto a destra. Mullis, Kary B. Mullis, Kary B. (Lenoir, North Carolina 1944), biochimico americano. Mise a punto la reazione a catena della polimerasi o PCR, innovativa tecnica di... Multimedia 2 elementi Vuoi altri contenuti di Encarta? Iscriviti subito e avrai a tua disposizione:
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65. Le Teorie Di Kary B. Mullis - Ecplanet.ch - Giovedì 15.9.2005 - Index
Translate this page Non si capisce allora perché susciti tanto kary B. mullis, Non c’è dubbio, kary B. mullis è il giullare che con lo sberleffo cerca di far crollare il re
http://www.ecplanet.com/canale/scienza-1/comunicazione-47/0/0/9235/it/ecplanet.r

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66. PCR.html
mullis, KB (2002). kary B. mullis—Nobel Lecture. Nobel eMuseum. http//www.nobel.se/ chemistry/laureates/1993/mullis-lecture.html (2002, Sept. 14).
http://www.macalester.edu/~montgomery/PCR.html
Polymerase Chain Reaction
Julia Adams
Research in Molecular Genetics
Technique of the Week
December 14, 2002
Definition

Reagents

Taq
Polymerase ...
References

Definition
Polymerase Chain Reaction (PCR) is a method for producing an extremely large number of copies of a specific DNA sequence through amplification. Reagents Taq polymerase has higher accuracy at lower concentrations. Magnesium chloride (MgCl ) is also essential for dNTP incorporation. The optimum concentration range for MgCl is 1.0-1.5 mM; low Mg leads to low PCR yields and an excess results in nonspecific products. Finally, Taq DNA polymerase is needed to polymerize the new DNA. Taq Polymerase Taq polymerse is from the thermophilic bacterium Thermus aquaticus , which was discovered by Thomas Brock in the 1960s in the hot springs of Yellowstone National Park Taq PCR Cycle The entire PCR cycle takes place in a thermocycler and is as follows:
  • Repeat process 20-30 times
The annealing temperature will vary depending on how well the primers match the desired sequence - the more perfect the match the higher the temperature. A common equation used for the determination of annealing temperature is T m = 2(A + T) + 4(G + C), where A, T, G, and C represent the numbers of each nucleotide in the primer sequence.

67. Nobel Prize In Chemistry 1993 - Press Release
Dr kary B. mullis, La Jolla, California, USA, for his invention of the polymerase The chemical methods that kary B. mullis and Michael Smith have each
http://www.nyx.net/~mjensen/nobel93.html

Press Release: The 1993 Nobel Prize in Chemistry
KUNGL. VETENSKAPSAKADEMIEN
THE ROYAL SWEDISH ACADEMY OF SCIENCES
13 October 1993
The Royal Swedish Academy of Sciences
has decided to award the 1993 Nobel Prize in Chemistry for contributions to the development of methods within DNA-based chemistry
with half to
Dr Kary B. Mullis , La Jolla, California, U.S.A., for his invention of the polymerase chain reaction (PCR) method,
and half to
Professor Michael Smith , University of British Columbia, Vancouver, Canada, for his fundamental contributions to the establishment of oligonucleotide-based, site-directed mutagenesis and its development for protein studies.
Decisive progress in gene technology through two new methods: the polymerase chain reaction (PCR) method and site-directed mutagenesis
The chemical methods that Kary B. Mullis and Michael Smith have each developed for studying the DNA molecules of genetic material have further hastened the rapid development of genetic engineering. The two methods have greatly stimulated basic biochemical research and opened the way for new applications in medicine and biotechnology.
The applications of Mullis' PCR method are already many. It is for example possible using simple equipment to multiply a given DNA segment from a complicated genetic material millions of times in a few hours, which is of very great significance for biochemical and genetic research. The method offers new possibilities particularly in medical diagnostics, and is used, for example, for discovering HIV virus or faulty genes in hereditary diseases. Researchers can also produce DNA from animals that became extinct millions of years ago by using the PCR method on fossil material.

68. Nobel Prizes In Molecular Biology
mullis, kary B., USA, La Jolla, CA, b. 1944. for his invention of the polymerase chain reaction (PCR) method ,. and by the other half to
http://home.sandiego.edu/~cloer/molecnobels.html
Selected Nobel Prizes in Molecular Biology
Official Nobel Website (San Diego Supercomputing Center mirror) Chemistry 1958 The prize was awarded to:
    SANGER, FREDERICK, Great Britain, Cambridge University, b. 1918:
"for his work on the structure of proteins, especially that of insulin". Nobel e-Museum Link Physiology or Medicine 1958 The prize was divided, one half being awarded jointly to:
    BEADLE, GEORGE WELLS, U.S.A., California Institute of Technology, Pasadena, CA, b. 1903, d. 1989; and TATUM, EDWARD LAWRIE, U.S.A., Rockefeller Institute for Medical Research, New York, NY, b. 1909, d. 1975:
"for their discovery that genes act by regulating definite chemical events"; and the other half to:
    LEDERBERG, JOSHUA, U.S.A., Wisconsin University, Madison, WI, b. 1925:
"for his discoveries concerning genetic recombination and the organization of the genetic material of bacteria". Nobel e-Museum Link Physiology or Medicine 1959 The prize was awarded jointly to:
    SEVERO OCHOA, U.S.A., New York University, New York; and ARTHUR KORNBERG, U.S.A., Stanford University, Stanford, CA;

69. SIDA Sin VIH: ¿Mito O Realidad?
930 1000 Myth and realities upon AIDS, kary B. mullis, PhD. kary B. mullis, PhD - Nobel Prize in Chemistry in 1993 for the discovery of the
http://free-news.org/cobruk01.htm
1st INTERNATIONAL SYMPOSIUM
ON ENIGMATIC AND UNCERTAIN SUBSTANCES AND MICROORGANISMS AIDS WITHOUT HIV:
MYTH OR REALITY?
AUDITORIUM LUIS A. CALVO
Universidad Industrial de Santander
Cra 27 Cll 9 - Ciudad Universitaria OCTOBER 2, 3, 4 and 5, 1997 BUCARAMANGA, COLOMBIA
SOUTH AMERICA PROGRAM Thursday October 2, 1997 8:00 a.m. to 10:00 a.m.
Registration SESSION A
Chairman: Fidias E. Leon-S, MD, PhD. 5:45 - 6:00 p.m. Opening Ceremony, Colombia National Anthem, State of Santander hymn, Santander Industrial University hymn.
Welcome by the Principal of University.
Welcome by the Dean of the School of Medicine.
Facts, Factors and distracters in human retroviruses: a personal view Fidias E. Leon-S, MD, PhD.
How much longer can we afford the AIDS virus monopoly? Peter Duesberg, PhD. Music presentation: UIS - Santander Industrial University Chorus. 8:15 p.m. Opening Reception. Friday October 3, 1997 SESSION B Chairman: Peter Duesberg, PhD, Secretary: Mauricio de Castro-Costa, MD, PhD. 7:30 - 8:00 a.m. Tropical spastic paraparesis without HTLV-1 Vladimir Zaninovic, MD. Discrepancy, evidence, coincidence, and inconsistency in the epidemiology of the tropical spastic paraparesis, Mauricio de Castro-Costa, MD, PhD.

70. Chain Reaction
Tech grad kary mullis selected Japan Prize Laureate. Dr. kary B. mullis, who developed one of the hottest techniques in biotechnology the polymerase chain
http://gtalumni.org/Publications/techtopics/spr93/chainreaction.html
Chain Reaction
Tech grad Kary Mullis selected Japan Prize Laureate D r. Kary B. Mullis, who developed one of the hottest techniques in biotechnology - the polymerase chain reaction - as been awarded the 1993 Japan Prize for Molecular and Cellular Technology in Medicine. A 1964 chemistry graduate of Georgia Tech, Mullis is a private consultant of nucleic acid chemistry. He will receive a cash award of $385,000 (50 million yen). The polymerase chain reaction (PCR) has revolutionized molecular biology, medicine and many other related scientific fields. PCR allows scientists to produce a specific DNA sequence without resorting to cloning. It is a way of amplifying specific DNA sequences from small amounts of a complex template. The Japan Prize was created by The Science and Technology Foundation of Japan to commend scientists and technologists throughout the world for major scientific and technological achievements that advance global peace and prosperity. After graduating from Tech, Mullis received a doctorate in biochemistry from the University of California, Berkely. Mullis worked for Cetus Corp., Emeryville, Calif. where he developed PCR. From 1986 to 1988, he was director of molecular biology at Xytronyx Inc. in San Diego, and began working as a consultant in 1987. The multiplying uses of PCR have kept his services as a consultant in demand. PCR can be used to determine which viruses are present in organs for transplant, and could help in the development of DNA for use as a drug, Mullis said in an article in the Winter 1992

71. Mullis A Vtereinaria - UniBo Magazine
Translate this page kary B. mullis, inventore del PCR, il procedimento per analizzare il DNA, adottato in tutto il mondo, è stato invitato dalla Facoltà di Medicina Vetrinaria
http://www.magazine.unibo.it/Magazine/Notizie/2003/03/31/mullis a vtereinaria.ht
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72. Buch.de - Bücher - Dancing Naked In The Mind Field - Kary B. Mullis
Translate this page Dancing Naked in the Mind Field - kary B. mullis Besorgungstitel versandfertig in 1 bis 2 Wochen EUR 14,95.
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Popular science from a Nobel Prize winner who applies his keen and eccentric mind to all manner of scientific assumptions. From astrology to mind-powered lightbulb switches, Mullis is brilliant, funny, provocative and charismatic. Perfect for fans of Mr Feynman.
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73. Nobelpreistraeger Kary B. Mullis Vip Promi Und Vip Linkliste Inkl. Stars & Stern

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74. HISTORIA
Translate this page kary B. mullis / Process For Amplifying Nucleic Acid Sequences Un resumen sobre la biografía de kary B. mullis, la historia del descubrimiento de la PCR y
http://www.espanol.pcrlinks.com/generalidades/historia.htm
Inicio PCR Generalidades Variantes Agregar link Buscar ... Ayuda GENERALIDADES HISTORIA Acerca de este sitio Inf or mación de c ... Términos y condiciones de uso

75. Storia Della Chimica. Premi Nobel Per La Chimica: Mullis
kary Banks mullis, una scheda biografica
http://www.minerva.unito.it/Theatrum Chemicum/NobelChimica/Mullis.htm
Theatrum Chemicum Personae: Kary B. Mullis Kary B. Mullis n. 1944 Premio Nobel per la chimica 1993 con Michael Smith Premio Nobel 1992 Premio Nobel 1994 Kary Banks Mullis, una scheda biografica Kary Banks Mullis nasce il 28 dicembre 1944 a Lenoir, nel North Carolina, da Cecil Banks Mullis e Bernice Barker Fredericks. Nel 1949 si trasferisce insieme ai genitori a Columbia, nel South Carolina. Nel 1962 si diploma alla Dreher High School di Columbia, dove è ben noto per il suo irriverente senso dell’umorismo, quanto per il fatto di essere straordinariamente portato per le materie scientifiche: nell’ultimo anno ricopre l’incarico di vicepresidente del corpo studentesco. Nel 1966 consegue il master in Chimica al Georgia Institute of Technology e si trasferisce alla University of California a Berkeley, dove nel 1972 ottiene il Ph.D. in Biochimica, materia che insegna fino all’anno successivo. Nel 1973 ottiene una borsa di studio post-dottorato in cardiologia pediatrica alla University of Kansas Medical School, occupandosi in particolare di angiotensina e della fisiologia vascolare del polmone. Nel 1977 si trasferisce per due anni alla University of California a San Francisco, per occuparsi di chimica farmaceutica. Due anni più tardi, nel 1979, è assunto dalla Cetus Corporation di Emeryville, California, come chimico esperto di DNA. Durante i sette anni che vi trascorre, si dedica alla ricerca sulla sintesi degli oligonucleotidi, e inventa la reazione a catena della polimerasi (PCR), per la quale riceve nel 1993 il premio Nobel per la Chimica.

76. THIS SEARCH THIS DOCUMENT THIS CR ISSUE GO TO Next Hit Forward
kary B. mullis SOUTH CAROLINA S 1993 NOBEL LAUREATE (Senate October 15, 1993) Mr. President, I rise to congratulate kary mullis on winning the 1993
http://thomas.loc.gov/cgi-bin/query/z?r103:S15OC3-373:

77. Kary Mullis - Wikipedia
Translate this page (Reindirizzamento da kary B. mullis) Nobel per la Chimica nel 1993, kary mullis è divenuto una leggenda per la scoperta della PCR (Polymerase Chain
http://it.wikipedia.org/wiki/Kary_B._Mullis
Kary Mullis
Da Wikipedia, l'enciclopedia libera.
(Reindirizzamento da Kary B. Mullis Nobel per la chimica Kary Banks Mullis (nato il 28 dicembre a Lenoir in North Carolina biochimico statunitense. Ha ottenuto il dottorato di ricerca a Berkeley nel . Vive in California con la moglie Nancy. Nobel per la Chimica nel 1993, Kary Mullis ¨ divenuto una leggenda per la scoperta della PCR (Polymerase Chain Reaction) una tecnica che ha rivoluzionato il mondo della chimica e della genetica, permettendo l’amplificazione in vitro di frammenti di DNA (il cosiddetto "test del DNA"), con innumerevoli applicazioni in campo medico, agrario, animale e nelle investigazioni della magistratura. Surfista e contestatore nella Berkeley negli anni 60 , Mullis ¨ un personaggio alquanto originale e discusso, che spesso si ¨ scontrato con le posizioni "ortodosse" della scienza ufficiale. ˆ noto per la sua critica alla teoria dell' HIV come origine dell' AIDS , per lo scetticismo rispetto alle cause del riscaldamento globale e del buco nell'ozono Nel suo libro del Ballando nudi nel campo della mente. Le idee (e le avventure) del pi¹ eccentrico tra gli scienziati moderni

78. TCS: Tech Central Station - What Is The Earth's 20th Century Temperature Trend?
The following important comments were made by kary B. mullis in his autobiography, Dancing Naked in the Mind Field. Science appeared in the seventeenth
http://techcentralstation.com/062404I.html
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The following important comments were made by Kary B. Mullis in his autobiography, "Dancing Naked in the Mind Field." Who is Kary Mullis? Mullis is the scientist and chemist that brought the world the gift of "all the DNA you wanted" by allowing copies to be made through "his invention of the polymerase chain reaction method." For that he was awarded 1/2 of the Nobel Prize in Chemistry in 1993 Mullis's comments have important ramifications for those of us involved in the scientific enterprise of investigating global warming by CO . Not only that we should be good enough to resist spreading unfounded fears (which is what I infer by merely browsing over what has been published over the last 10 years or so) of a globally warm Earth; but we better be sure that what we say and publish our scientific results are accurate and at least can be subjected to the minimal scientific standard of being reproducible.

79. Weird Science
The prosecution had a videotape of kary mullis on Rollerblades in La Jolla, and a mom who gave good advice ( Now kary B., don t you blow your eyes out!
http://www.nytimes.com/books/98/10/11/reviews/981011.11teresit.html

80. Chinadaily Community
Nobel Prize winner and future OJ Simpson trial DNA expert witness kary B. mullis spoke on campus April 6 in the Small Auditorium of the University Student
http://bbs.chinadaily.com.cn/forumpost1.shtml?pid=333850

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