APPLICATIONS COMPANY CATALOG Working Procedures in Microwave Histology by Vincent R. Klump, Jr., HT (ASCP), Histology Services, East Haven, CT note: Mr. Klump was instrumental in the development of a standardized, consistent, reproducible protocol for microwave histoprocessing in the original Energy Beam Sciences H2500 Microwave Processor. Contents: Microwave Tissue Processing Antigen Retrieval Special Stains Microwave Stimulated Decalcification ... Microwave Tissue Processing QC Chart Microwave Tissue Processing Tissue criteria: Maximum tissue size for this procedure: 3mm x 3mm x 3mm. Variations of this size can also be accommodated, but total tissue volume should not exceed 3mm cubed. Examples: 3mm punch biopsies, core needle biopsies, core bone marrow biopsies, shave biopsies. Tissue should be fixed in 10% NB Formalin for a minimum of four hours. Equipment: - Laboratory microwave equipped with a temperature probe accurate to +/- 1ÂșC Microwave-safe container holding a minimum of 250ml (optional) microwave-safe cassette rack supplied by Energy Beam Sciences 100% ethanol 100% isopropanol liquid paraffin boiling chips (preferably, common stones not bigger than 1cm x .5cm x .5cm)
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